suc2-, that cannot grow on sucrose. Both mutations are linked tothepyrA gene by P1
transduction. While the cotransduction of suc1 and suc2 is 95%,thecotransduction of
pyrA and suc2 is 60%.
To determine the relative order of pyrA, suc1 and suc2, youperformthe following two
experiments:
Experiment 1: P1 grown on a pyrA+ suc1- is used to infectpyrA-suc2-.
Of the pyrA+ transductants, 5% are suc+
Experiment 2: P1 grown on a pyrA+ suc2- is used to infectpyrA-suc1-.
Of the pyrA+ transductants, 0.1% are suc+.
a. (5 points) Draw a map showing the order of the threemutations.Which mutation,
suc1 or suc2 is closer to pyrA?
b. (8 points) How many crossovers were required in getting suc+ineach experiment?
c. (7 points) An Hfr strain was isolated that transfers galBearlyand pyrA and suc1 late.
From the Hfr an F’ plasmid is isolated, and it transfers suc1butnot galB or pyrA. Draw
a map of the relevant portion of the chromosome in theHfr,including the position and
orientation of the origin its transfer.
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